Anti-Fibrinogen alpha chain (FGA) Polyclonal Antibody for WB, IHC-P, ELISA - P02671

Core Product Specifications and Parameters

Biological Background: Fibrinogen alpha chain Function and Localization

• Fibrinogen alpha chain is cleaved by thrombin to yield fibrin monomers, which polymerize with fibrinogen beta and gamma to form an insoluble fibrin matrix, a primary component of blood clots.
• Besides hemostasis, fibrin functions during early wound repair to stabilize the lesion and guide cell migration during re-epithelialization.
• Contrary to earlier in vitro studies, fibrinogen is not absolutely required for platelet aggregation or thrombus formation in vivo, but enhances SELP expression on activated platelets via an ITGB3-dependent pathway.
• Maternal fibrinogen is essential for successful pregnancy, and fibrin deposition is associated with infection, where it protects against IFNG-mediated hemorrhage and facilitates innate and T-cell mediated immune responses.
• The protein is secreted and predominantly detected in blood plasma.
• Relevant keywords include blood coagulation, hemostasis, adaptive immunity, innate immunity, amyloidosis, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning amino acids 410-559 of human FGA. Consider that the antibody may detect full-length FGA and potentially cross-react with isoforms or degradation fragments.
• For Western blot (WB), the predicted molecular weight is approximately 95 kDa. Optimize loading controls and validate specificity using relevant positive and negative lysates.
• In immunohistochemistry (IHC-P), consider appropriate antigen retrieval methods and validate staining in tissues known to express FGA (e.g., liver, plasma).
• For ELISA, this polyclonal antibody can be explored as a capture or detection reagent; pairing with a monoclonal antibody may improve assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined one-stop sourcing channel for IVD raw materials. This Fibrinogen alpha chain (FGA) polyclonal antibody supports research into hemostasis, thrombosis, and wound healing. Beyond this product, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your development from concept to clinic.