Anti-Fibrillarin/U3 RNP Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P22087

Core Product Specifications and Parameters

Biological Background: Fibrillarin/U3 RNP Function and Localization

• Fibrillarin is an rRNA 2'-O-methyltransferase, also known as 34 kDa nucleolar scleroderma antigen, histone-glutamine methyltransferase, and U6 snRNA 2'-O-methyltransferase fibrillarin.
• It catalyzes site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA, guided by box C/D snoRNAs. Related references: PMID:30540930
• Functions as a protein methyltransferase by methylating histone H2A at glutamine 105 (H2AQ104me), which impairs FACT complex binding at the 35S ribosomal DNA locus. Related references: PMID:24352239, PMID:30540930
• Methylates U6 snRNA at specific 2'-O sites within box C/D RNP complexes, a modification essential for mRNA splicing fidelity. Related references: PMID:32017898
• Acts as an integral component of the small subunit (SSU) processome, the first precursor of the small ribosomal subunit, and is critical for ribosome biogenesis. Related references: PMID:34516797
• Predominantly localizes to the nucleolus and nucleoplasm of the nucleus.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitin conjugation; it is also methylated itself and contains RNA-binding domains.

Experimental Guidance and Technical Tips

• For western blot, a band approximately 34 kDa is expected; use appropriate molecular weight markers for confirmation.
• The immunogen region (amino acids 222-321) shows conservation across human, mouse, and rat, supporting the observed cross-reactivity; validate performance in your specific sample system.
• In immunofluorescence or ICC, nucleolar localization should be evident; optimize fixation and permeabilization conditions for nuclear target detection.
• For immunoprecipitation, verify antibody compatibility with your extraction buffer and consider including a no-antibody control.
• For ELISA applications, assess coating conditions and use a known positive control such as the immunogen peptide to establish assay parameters.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and academic institutes with efficient access to IVD raw materials, including validated antibodies and ancillary reagents for nucleolar protein and ribosome biogenesis research. Our sourcing platform streamlines procurement from concept to clinic, offering optimized monoclonal/polyclonal antibodies, bulk reagents, and rare target raw-material supply to accelerate your assay development and validation workflows.