Anti-Fibrillarin/U3 RNP Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P22087

Core Product Specifications and Parameters

Biological Background: Fibrillarin/U3 RNP Function and Localization

• Fibrillarin is an S-adenosyl-L-methionine-dependent methyltransferase with dual specificity for RNA and protein substrates. Related references: PMID:24352239 PMID:30540930 PMID:32017898
• It catalyzes site-specific 2'-O-methylation of pre-ribosomal RNA guided by box C/D snoRNAs, essential for pre-rRNA processing. Related references: PMID:30540930
• Fibrillarin also methylates U6 snRNA, a modification critical for mRNA splicing fidelity. Related references: PMID:32017898
• As a protein methyltransferase, fibrillarin methylates histone H2A at Gln-105 (H2AQ104me), which impairs FACT binding and is enriched at the 35S rDNA locus. Related references: PMID:24352239 PMID:30540930
• Fibrillarin is a component of the small subunit (SSU) processome, the earliest precursor of the small ribosomal subunit, playing a central role in ribosome biogenesis. Related references: PMID:34516797
• The protein localizes predominantly to the nucleolus and nucleoplasm, consistent with its roles in rRNA processing and ribosome assembly.
• Known also as the 34 kDa nucleolar scleroderma antigen, fibrillarin is targeted by autoantibodies in systemic sclerosis, underscoring its immunogenicity and clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-321 of human fibrillarin, covering a central domain; consider this when planning epitope-dependent experiments.
• For western blot (WB), the expected band is approximately 34 kDa; validate using positive control lysates (e.g., HeLa, Jurkat) and appropriate loading controls.
• For immunofluorescence (IF/ICC), expect nucleolar and nucleoplasmic staining; co-staining with nucleolar markers may aid verification.
• In immunoprecipitation (IP), this polyclonal antibody may enrich the SSU processome complex; cross-linking optimization may be required.
• As a rabbit polyclonal antibody, lot-to-lot variability may exist; consider routine validation for critical applications.
• Store antibody as recommended upon receipt (ice bag shipping) to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, technical services, and consulting. Our anti-fibrillarin polyclonal antibody is an excellent choice for studies on ribosome biogenesis, RNA methylation, and autoimmune disease research. As your sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—contact us to streamline your procurement from concept to clinic.