Anti-Fibrillarin Rabbit Monoclonal Antibody for WB - P22087

Core Product Specifications and Parameters

Biological Background: Fibrillarin Function and Localization

• Fibrillarin is an S-adenosyl-L-methionine-dependent methyltransferase that methylates both RNAs and proteins.
  Related references: PMID:24352239, PMID:30540930, PMID:32017898
• It catalyzes site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA, guided by box C/D snoRNAs.
  Related references: PMID:30540930
• Fibrillarin also methylates U6 snRNA, a modification essential for proper mRNA splicing.
  Related references: PMID:32017898
• Acts as a histone methyltransferase by specifically methylating Gln-105 of histone H2A (H2AQ104me), impairing FACT complex binding and regulating rDNA transcription.
  Related references: PMID:24352239, PMID:30540930
• Fibrillarin is a component of the small subunit (SSU) processome, essential for the assembly of the small ribosomal subunit.
  Related references: PMID:34516797
• It primarily localizes to the nucleolus and nucleoplasm.
• Fibrillarin is subject to multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen peptide (aa 111-210) targets the central domain of Fibrillarin; for cross-reactivity concerns, verify sequence homology in your species of interest.
• For Western blotting, a single band near 34 kDa is expected; use appropriate positive control lysates (e.g., HeLa, HEK293).
• In immunofluorescence or immunohistochemistry, predominant nucleolar staining should be observed; co-localization with nucleolar markers such as NPM1 is recommended.
• For immunoprecipitation, note that Fibrillarin is part of large ribonucleoprotein complexes; optimize lysis and wash conditions to preserve interactions if studying binding partners.
• For ELISA applications, test linear range with recombinant Fibrillarin or cell/tissue extracts; consider using the antibody in a capture-detection pair format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one-stop procurement channel for IVD raw materials, from concept to clinic. This Fibrillarin antibody supports nucleolar biology and ribosome biogenesis research, and we can supply matched antibody pairs, bulk reagents, and customized sourcing for your next assay development project. Reach out to explore our full portfolio and accelerate your biomarker workflow.