Anti-FIBP Rabbit Polyclonal Antibody for WB, IHC-P - O43427

Core Product Specifications and Parameters

Biological Background: Acidic Fibroblast Growth Factor Intracellular-Binding Protein Function and Localization

• The FIBP gene encodes acidic fibroblast growth factor intracellular-binding protein (FGF-1 intracellular-binding protein), which may exist in multiple isoforms due to alternative splicing.
• FIBP is implicated in the mitogenic signaling of fibroblast growth factor 1 (FGF1) and may cooperate with IER2 to regulate FGF signaling during embryonic left-right asymmetry establishment.
• The protein localizes to the nucleus and the endomembrane system, consistent with its intracellular role in signal transduction.
• Post-translational modifications include acetylation; the protein is also associated with membrane components and has been identified in proteomics screens.
• FIBP exhibits tissue-specific expression, with high levels in heart, skeletal muscle, and pancreas, moderate expression in brain, and lower presence in placenta, liver, and kidney.
• The gene is listed as a reference proteome member and has variants linked to disease, underscoring its biomedical significance.
• The predicted molecular weight is approximately 42 kDa (UniProt: 41.9 kDa), consistent with the observed band in western blot applications.

Experimental Guidance and Technical Tips

• For western blot (WB), consider using a dilution range of 1:500–1:2000 and validate with a positive control lysate from tissues with known high expression (e.g., heart, skeletal muscle).
• In immunohistochemistry (IHC-P), antigen retrieval may be required; testing on paraffin-embedded sections of pancreas or heart is recommended.
• For immunoprecipitation (IP), the antibody may capture native FIBP; confirm efficiency by western blot analysis.
• In ELISA, pairing with a suitable detection antibody could enable quantitative assays; validate using recombinant FIBP protein as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials. This anti-FIBP polyclonal antibody, targeting a key mediator of FGF signaling, is part of our expanding catalog for developmental and cancer research. In addition to single reagents, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development.