Anti-FHL5 Rabbit Polyclonal Antibody for WB, ELISA - Q5TD97

Core Product Specifications and Parameters

Biological Background: FHL5 Function and Localization

• FHL5, also known as Activator of CREM in testis (ACT), is a member of the four and a half LIM domains protein family.
• It functions as a transcriptional coactivator that stimulates CREM transcriptional activity in a phosphorylation-independent manner.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• FHL5 expression is testis-specific at the protein level, suggesting a specialized function in spermatogenesis.
• It contains multiple LIM domains, which are zinc-finger motifs that mediate protein-protein interactions and metal-ion binding.
• The LIM domains enable FHL5 to interact with various partners, potentially linking signaling pathways to gene expression.
• As a reference proteome member and identified by proteomics, FHL5 is a well-characterized protein with structural data available.
• Given its testis-restricted expression and CREM activation, FHL5 may serve as a key regulator in male germ cell development.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human FHL5 protein (aa 1-284), so the polyclonal antibody may recognize multiple epitopes; consider it for both WB and ELISA applications.
• For Western blot, FHL5 has a predicted molecular weight of approximately 33 kDa; validate detection in the appropriate sample system, such as human or mouse testis lysates.
• Due to high tissue specificity, ensure the expression of FHL5 in your sample type; testis-derived samples are recommended as positive controls.
• For ELISA, the antibody can be used for detection or capture; include appropriate controls and optimize coating conditions.
• Cross-reactivity with mouse FHL5 is expected based on sequence homology; validation in mouse models may be relevant.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop procurement solution for IVD raw materials, technical services, and consulting to support your diagnostic and research workflows. This anti-FHL5 polyclonal antibody exemplifies our portfolio of optimized polyclonal and monoclonal antibodies suitable for reproductive biology and transcriptional regulation studies. Beyond individual antibodies, we provide validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to meet your complete assay development needs.