Anti-FHL5 Monoclonal Antibody for WB, IF/ICC, IP, ELISA - Q5TD97

Core Product Specifications and Parameters

Biological Background: FHL5 Function and Localization

• FHL5 (Four and a half LIM domains protein 5), also known as Activator of cAMP-responsive element modulator in testis (ACT), is a LIM domain-containing protein encoded by the FHL5 gene.
• It may play a role in spermatogenesis by stimulating CREM (cAMP-responsive element modulator) transcriptional activity in a phosphorylation-independent manner.
• The protein contains four and a half LIM domains, which are cysteine-rich zinc-finger motifs that mediate protein-protein interactions and are essential for its function.
• FHL5 is primarily localized to the nucleus, consistent with its role as a transcriptional co-activator in testicular germ cells.
• Its expression is testis-specific at the protein level, indicating a specialized function in male reproductive biology.
• UniProtKB annotations classify FHL5 under keywords such as LIM domain, metal-binding, nucleus, and zinc-finger, highlighting its structural and functional characteristics.
• The calculated molecular weight is approximately 32.7 kDa (UniProt), consistent with the observed 33 kDa band in immunoblotting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 162–284 (C-terminal region) of human FHL5, which may enable specific detection of the full-length protein.
• Supplied as rabbit monoclonal antibody, suitable for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), immunoprecipitation (IP), and ELISA as indicated by the manufacturer.
• Cross-reactivity with mouse makes this antibody a candidate for comparative studies in human and mouse testis lysates or tissue sections.
• For WB, consider using nuclear-enriched extracts from testis tissue; include species-matched positive controls and validate specificity with knockdown or knockout models as needed.
• In IF/ICC, anticipate nuclear staining; optimize fixation and permeabilization conditions for the cell type or tissue under investigation.

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