Anti-FHL3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q13643

Core Product Specifications and Parameters

Biological Background: FHL3 Function and Localization

• FHL3 (Four and a half LIM domains protein 3), also known as SLIM2 (Skeletal muscle LIM-protein 2), is a member of the FHL family of LIM domain proteins.
• Acts as a transcriptional coactivator: recruited by SOX15 to FOXK1 promoters to enhance FOXK1-mediated transcription.
• Localizes to both the nucleus and cytoplasm.
• Expressed exclusively in skeletal muscle tissue.
• Contains LIM domains that function as zinc-binding motifs, involved in metal binding and transcription regulation.
• Post-translationally modified by acetylation, as identified by proteomics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human FHL3 (amino acids 1–280); antibody reactivity may cover the entire protein. Validate in human skeletal muscle lysates for WB.
• For IF/ICC, optimize permeabilization conditions to detect FHL3 in both nuclear and cytoplasmic compartments.
• Reported cross-reactivity with mouse indicates suitability for mouse skeletal muscle studies; confirm by comparative analysis.
• The predicted molecular weight is 31kDa; verify band size by using appropriate molecular weight markers in your system.
• As dilutions are not provided, titrate the antibody under your specific conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For skeletal muscle transcriptional regulation research, we offer validated antibodies such as this anti-FHL3 polyclonal antibody, along with antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing solutions. Our B2B focus ensures reliable supply and technical support for your immunoassay development needs.