Anti-FHL2 Monoclonal Antibody for WB, ELISA - Q14192

Core Product Specifications and Parameters

Biological Background: FHL2 Function and Localization

• FHL2 (Four and a half LIM domains protein 2), also known as DRAL, AAG11, SLIM3, is a LIM domain protein that functions as a molecular transmitter linking various signaling pathways to transcriptional regulation.
• It negatively regulates the transcriptional repressor E4F1, potentially influencing cell growth.
• FHL2 inhibits FOXO1 transcriptional activity and its apoptotic function by enhancing FOXO1 interaction with SIRT1 and subsequent deacetylation.
• Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes. Related references: PMID:28717008
• The protein contains LIM domains, which are zinc-finger motifs mediating protein-protein interactions, and is subject to alternative splicing, phosphorylation, and ubiquitination.
• It localizes to the cytoplasm, nucleus, and specifically to the Z line of sarcomeres in muscle cells, reflecting its role in muscle biology.
• Tissue specificity: Predominantly expressed in skeletal muscle and heart.

Experimental Guidance and Technical Tips

• The recombinant immunogen spans amino acids 50–260 of human FHL2, encompassing key LIM domains; this region may ensure specificity for FHL2 across reactive species (human, mouse, rat).
• For Western blot, expect a band at approximately 32 kDa. Due to potential phosphorylation and other modifications, minor shifts or additional bands may be observed; validate using positive controls from skeletal muscle or heart tissues.
• For ELISA, validate the antibody’s reactivity with the target protein in the relevant sample matrix and consider a blocking strategy to reduce non-specific binding.
• If cross-reactivity with other FHL family members is a concern, verify specificity by including appropriate controls or using knockdown/knockout models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive supplier of IVD raw materials, offering one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. The FHL2 antibody is particularly relevant to research and assay development in transcriptional regulation, muscle biology, and cardiac signaling pathways. Beyond standard catalog products, CamelBio supports rare target raw-material sourcing and can assist from concept to clinic, making it a reliable partner for diagnostic manufacturers and research labs.