Anti-FHIT Polyclonal Antibody for WB, ELISA - P49789

Core Product Specifications and Parameters

Biological Background: FHIT Function and Localization

• FHIT (Fragile histidine triad protein) is a member of the histidine triad (HIT) superfamily and functions as a dinucleoside triphosphate hydrolase, primarily catalyzing the hydrolysis of diadenosine triphosphate (Ap3A) to AMP and ADP (PubMed:12574506, PubMed:15182206, PubMed:8794732).

  Related references: PMID:12574506 PMID:15182206 PMID:8794732

• It also exhibits adenylylsulfatase activity (PubMed:18694747) and adenosine 5'-monophosphoramidase activity (PubMed:18694747), and can function as an adenylylsulfate-ammonia adenylyltransferase (PubMed:26181368).

  Related references: PMID:18694747 PMID:26181368

• FHIT acts as a tumor suppressor by modulating CTNNB1 transcriptional activation (PubMed:18077326), inducing apoptosis via SRC and AKT1 pathways (PubMed:16407838), and inhibiting MDM2-mediated degradation of p53/TP53 (PubMed:15313915).

  Related references: PMID:18077326 PMID:16407838 PMID:15313915

• The protein localizes to the cytoplasm, mitochondria, and nucleus. The mitochondrial form sensitizes Ca²⁺ transporters, enhancing mitochondrial calcium uptake to promote apoptosis (PubMed:19622739).

  Related references: PMID:19622739

• FHIT is expressed at low levels in all tissues, with phosphorylated FHIT detected in liver and kidney but not brain, lung, or tumor cell lines.

• It is frequently deleted or altered in cancers due to its location at the fragile site FRA3B, and its expression is associated with chromosomal rearrangement.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 25–147, representing nearly the full-length FHIT protein, which suggests broad epitope coverage.

• For Western blot, the expected band is approximately 17 kDa; use a suitable percentage gel and validate with positive and negative controls (e.g., known FHIT-expressing and deficient cell lines).

• When performing ELISA, consider using the recombinant protein immunogen as a positive control to establish standard curves.

• Since the antibody cross-reacts with mouse FHIT, verify homology in the immunogen region between species for quantitative comparisons.

• FHIT may be present in multiple subcellular compartments; fractionation or immunofluorescence can provide localization data.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined sourcing solution for high-quality IVD raw materials. This anti-FHIT polyclonal antibody is part of our comprehensive portfolio supporting cancer research and nucleotide metabolism studies. From antibody pairs to bulk reagents, CamelBio bridges the gap between concept and clinic.