Anti-FH (Fumarate Hydratase) Polyclonal Antibody for WB, ELISA - P07954

Core Product Specifications and Parameters

Biological Background: FH (Fumarate Hydratase) Function and Localization

• FH catalyzes the reversible hydration of fumarate to L-malate in the TCA cycle, a key step in mitochondrial energy production (PubMed:30761759). Related references: PMID:30761759

• Cytosolic FH isoforms participate in the urea cycle and arginine metabolism, producing fumarate as a by-product (By similarity).

• FH is annotated as a tumor suppressor and disease variant protein; mutations are linked to hereditary cancer syndromes such as HLRCC.

• In response to DNA damage, FH is phosphorylated by PRKDC and translocates to the nucleus, where it promotes NHEJ repair by inhibiting KDM2B demethylase, leading to increased H3K36me2 at DSBs (PubMed:20231875, PubMed:26237645). Related references: PMID:20231875, PMID:26237645

• FH localizes primarily to mitochondria but also to the cytosol; nuclear accumulation occurs after DNA damage (PubMed:26237645).

• Alternative initiation produces different isoforms that may favor either hydration or dehydration reactions (Probable).

• FH is expressed in red blood cells and is underexpressed in patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.

• Post-translational modifications include acetylation and phosphorylation; PRKDC-mediated phosphorylation at DSBs is critical for its DNA repair role.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–150 of human FH; consider using positive controls such as HeLa or HEK293T lysates for WB.
• For Western blotting, a specific band at ~55 kDa should be observed; use appropriate loading controls and molecular weight markers.
• Subcellular fractionation may improve detection of mitochondrial or nuclear FH pools.
• For ELISA, optimize coating antigen and antibody titration to achieve best signal-to-noise ratios.
• Cross-reactivity with mouse and rat FH allows multispecies studies; validate in your experimental system.
• Pre-adsorption with the immunizing peptide can confirm band specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research labs, offering one-stop access to IVD raw materials and technical support from concept to clinic. With expertise in antibody pair validation, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, we can streamline your FH-focused research in cancer metabolism and mitochondrial diseases. Whether you need rare-target antibodies or complete reagent sets, CamelBio provides reliable, scalable sourcing solutions tailored to your development pipeline.