Anti-FGR Polyclonal Antibody for WB, IHC-P, ELISA - P09769

Core Product Specifications and Parameters

Biological Background: Tyrosine-protein Kinase Fgr Function and Localization

• FGR (Tyrosine-protein kinase Fgr) is a non-receptor tyrosine kinase belonging to the SRC family, with aliases SRC2, c-fgr, p55-Fgr, p58-Fgr, and p58c-Fgr.
• It transmits signals from cell surface receptors lacking intrinsic kinase activity, regulating immune cell functions in neutrophils, monocytes, macrophages, and mast cells.
• Subcellularly, FGR localizes to the cell membrane, ruffle membrane, cytosol, cytoskeleton, and mitochondrial compartments (inner membrane and intermembrane space).
• FGR plays a key role in actin cytoskeleton remodeling, promoting cell spreading, adhesion, and migration via RAC1 activation downstream of integrins ITGB1/ITGB2.
• It acts downstream of immunoreceptors such as MS4A2/FCER1B, FCGR2A, and FCGR2B, phosphorylating targets like SYK, PLD2, and CBL to activate MAP kinase and AKT pathways.
• Tissue expression is prominent in neutrophils, monocytes, natural killer cells, and large lymphocytes.
• As a proto-oncogene, FGR is homologous to the v-fgr oncogene and is subject to lipid modifications (myristoylation, palmitoylation), phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human FGR; the resulting polyclonal antibody may recognize multiple epitopes.
• For Western blot, the predicted molecular weight of FGR is approximately 59 kDa; consider using appropriate positive controls (e.g., immune cell lysates) and validate band specificity.
• For IHC-P and ELISA applications, it is advisable to perform antigen retrieval and test optimal antibody dilutions using tissues or samples known to express FGR.
• As a polyclonal antibody, lot-to-lot variability is possible; always validate performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting for diagnostic manufacturers and research laboratories. Our anti-FGR polyclonal antibody supports the study of immune signaling and kinase biology, and we offer bulk sourcing, validated antibody pairs, and customized solutions for rare target procurement. Partner with CamelBio to streamline your development from concept to clinic.