Anti-FGL2 Polyclonal Antibody for WB and ELISA - Q14314

Core Product Specifications and Parameters

Biological Background: Fibroleukin (FGL2) Function and Localization

• FGL2, also known as Fibroleukin or fibrinogen-like protein 2 (pT49), is a member of the fibrinogen-related protein family and functions as a secreted immune regulator.
• It is proposed to play a role in physiologic lymphocyte functions, particularly at mucosal sites, suggesting involvement in mucosal immunity and T-cell regulation.
• Constitutively expressed in cytotoxic T-cells, Fibroleukin may contribute to the effector functions or regulation of these cells.
• The protein features a signal peptide directing secretion, and contains coiled-coil domains and disulfide bonds, which are important for its structural integrity and interaction.
• As a glycoprotein, post-translational glycosylation likely influences its stability and bioactivity.
• Subcellular localization is predominantly extracellular (secreted), consistent with its role in intercellular communication within the immune system.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band at approximately 50 kDa is expected; consider using reducing conditions and include appropriate positive controls such as human cytotoxic T-cell lysates or recombinant FGL2 protein.
• ELISA applications may be optimized by coating with the recombinant immunogen or capturing native Fibroleukin from cell culture supernatants or biological fluids.
• Since Fibroleukin is secreted, analyzing concentrated conditioned media from activated T-cells could enhance detection sensitivity.
• Always validate the antibody in your specific sample matrix and experimental setup to determine optimal working conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing for diagnostic manufacturers and research institutes, providing one-stop access to validated antibodies, antigens, and ancillary reagents. The FGL2 Rabbit pAb supports investigations into mucosal immunity and T-cell biology. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonals/polyclonals, bulk reagents, and rare target sourcing to accelerate your assay development and manufacturing workflows.