Anti-FGG Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P02679

Core Product Specifications and Parameters

Biological Background: Fibrinogen gamma chain Function and Localization

• FGG encodes the gamma chain of fibrinogen, a glycoprotein that together with alpha (FGA) and beta (FGB) chains polymerizes to form insoluble fibrin matrix, essential for blood clot formation.
• The protein has a major function in hemostasis as a primary component of blood clots and also stabilizes wounds during early repair by guiding cell migration during re-epithelialization.
• In addition to hemostasis, it enhances expression of SELP on activated platelets through an ITGB3-dependent pathway, though it is not absolutely required for thrombus formation in vivo.
• Maternal fibrinogen is essential for successful pregnancy, and fibrin deposition protects against IFNG-mediated hemorrhage during infection, while also facilitating antibacterial immune responses via innate and T-cell pathways.
• Fibrinogen gamma chain is secreted and detected in blood plasma.
• The gene undergoes alternative splicing, and the mature protein features post-translational modifications including phosphorylation, sulfation, and disulfide bond formation; it binds calcium and contains coiled-coil domains.
• Disease variants of FGG are associated with bleeding disorders, and its cross-linking via isopeptide bonds is critical for clot stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 169-433 of human FGG, a region near the C‑terminus. This design may recognize both full‑length and certain splice variants; confirm specificity in your sample matrix.
• For Western blot, expect bands around 49 kDa and 52 kDa depending on post‑translational modifications; always include appropriate loading controls and validate with untreated vs. treated samples.
• In immunofluorescence/ICC, optimize permeabilization steps for detection of secreted protein; paraformaldehyde fixation is often compatible.
• For IHC‑P, test antigen retrieval methods (e.g., heat‑mediated citrate pH 6.0) and validate with positive tissue controls such as placenta or liver.
• Direct ELISA can be employed for quantitative detection of fibrinogen in plasma or purified samples; ensure antibody coating meets assay requirements.
• Cross‑reactivity is reported for human; if used in other species, perform validation experiments in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides convenient one‑stop access to IVD raw materials like this anti‑FGG rabbit monoclonal antibody, supporting diagnostic and research applications in thrombosis, hemostasis, and wound healing. We offer validated antibody pairs, bulk ancillary reagents, and can source rare‑target raw materials to accelerate your assay development from concept to clinic.