Anti-FGFR4 Polyclonal Antibody for WB, ELISA - P22455

Core Product Specifications and Parameters

Biological Background: FGFR4 Function and Localization

• FGFR4 is a fibroblast growth factor receptor and tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors, regulating cell proliferation, differentiation, and migration.
• It plays a critical role in metabolic regulation, including lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism, and phosphate homeostasis. FGFR4 is required for the normal down-regulation of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19.
• Ligand binding triggers activation of multiple signaling cascades: phosphorylation of PLCG1 generates diacylglycerol and inositol 1,4,5-trisphosphate; phosphorylation of FRS2 recruits GRB2, GAB1, PIK3R1, and SOS1, leading to activation of RAS, MAPK1/ERK2, MAPK3/ERK1, and the AKT1 pathway.
• FGFR4 signaling is down-regulated by receptor internalization and degradation, a process promoted by SRC-dependent phosphorylation of the matrix protease MMP14 and its subsequent lysosomal degradation.
• Subcellular localization includes the cell membrane, endosomes, endoplasmic reticulum, and a secreted form.
• It is expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
• Post-translational modifications include glycosylation, phosphorylation, and ubiquitin conjugation. The protein undergoes alternative splicing and contains immunoglobulin-like domains, a kinase domain, and a transmembrane helix.
• Gene aliases include TKF, JTK2, and CD334.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22–150 in the extracellular domain; the resulting polyclonal antibody may preferentially recognize epitopes in the N-terminal region, which is useful for detecting cell surface FGFR4.
• In Western blotting (WB), a band around 88 kDa is expected; however, glycosylation and other modifications may cause slight shifts in apparent molecular weight. Validate the antibody in your specific sample system.
• For ELISA applications, consider pairing this antibody with a suitable capture antibody if developing a sandwich format. Perform cross-reactivity testing against mouse and rat samples as indicated, and include appropriate negative controls.
• Recommended starting dilutions and optimal conditions should be determined empirically by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. This FGFR4 polyclonal antibody is ideal for studies in cancer signaling, metabolic disorders, and receptor biology. As a trusted sourcing partner, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your assay development.