Anti-FGFR3 Rabbit Monoclonal Antibody for IHC-P and ELISA - P22607

Core Product Specifications and Parameters

Biological Background: FGFR3 Function and Localization

• FGFR3 (Fibroblast growth factor receptor 3) is a tyrosine-protein kinase that serves as a cell-surface receptor for fibroblast growth factors, playing a critical role in regulating cell proliferation, differentiation, and apoptosis.
• It is essential for normal skeleton development, regulating chondrocyte differentiation, proliferation, and apoptosis, as well as osteogenesis and postnatal bone mineralization by osteoblasts.
• FGFR3 signaling activates multiple downstream cascades, including the PLCG1 pathway (producing diacylglycerol and inositol 1,4,5-trisphosphate) and the RAS-MAPK and AKT1 pathways via phosphorylation of FRS2 and recruitment of GRB2, GAB1, PIK3R1, and SOS1.
• The protein localizes primarily to the cell membrane but is also found in cytoplasmic vesicles, the endoplasmic reticulum, and a secreted isoform (isoform 3) that can bind FGFs and potentially interfere with signaling.
• FGFR3 is expressed in brain, kidney, and testis, with high expression in fetal kidney, lung, small intestine, and brain; isoform 2 is detected in epithelial cells, while isoforms 1 and 2 are present in fibroblastic cells.
• Mutations causing constitutive kinase activation or impaired maturation and degradation lead to aberrant signaling and are associated with skeletal dysplasias (e.g., dwarfism), craniosynostosis, and cancer.
• The protein undergoes alternative splicing, producing isoforms with distinct tissue distributions and functional properties, and is subject to post-translational modifications including phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide from the N‑terminal region (amino acids 1–100) of human FGFR3, which may target an extracellular domain; consider this when designing blocking or competition experiments.
• For IHC‑P, use formalin‑fixed paraffin‑embedded human tissue sections; antigen retrieval conditions (e.g., heat‑induced epitope retrieval) should be optimized for the target epitope and tissue type.
• In ELISA applications, this rabbit monoclonal antibody can be employed as a detection reagent; pairing with a capture antibody against a non‑overlapping epitope may improve sensitivity.
• Cross‑reactivity is indicated only for human FGFR3; reactivity with other species or related FGFR family members (e.g., FGFR1, FGFR2) has not been reported and should be validated if needed.
• As a rabbit monoclonal antibody, it offers high specificity and lot‑to‑lot consistency; however, optimal working dilutions for each assay must be determined empirically by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high‑quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This FGFR3 rabbit mAb supports studies in skeletal development, oncology, and cell signaling, and we can assist with sourcing rare‑target reagents and custom development to accelerate your projects. Reach out to discuss how our end‑to‑end supply capabilities can benefit your next diagnostic or research endeavor.