Anti-FGFR3 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P22607

Core Product Specifications and Parameters

Biological Background: FGFR3 Function and Localization

• FGFR3 (Fibroblast Growth Factor Receptor 3) is a receptor tyrosine kinase that binds fibroblast growth factors, thereby regulating key cellular processes such as proliferation, differentiation, and apoptosis.
• It plays an essential role in chondrocyte differentiation and skeletal development; FGFR3 is required for normal bone mineralization by osteoblasts and regulates both osteogenesis and postnatal bone remodeling.
• Upon ligand binding, FGFR3 triggers multiple intracellular signaling cascades, including the MAPK/ERK (via phosphorylation of FRS2 and recruitment of GRB2, SOS1) and AKT1 pathways, as well as PLCG1-mediated production of second messengers diacylglycerol and inositol 1,4,5-trisphosphate.
• FGFR3 is also involved in the regulation of vitamin D metabolism.
• Subcellular localization includes the cell membrane, cytoplasmic vesicles, and the endoplasmic reticulum; a secreted isoform (isoform 3) exists that retains FGF-binding capacity and may modulate signaling.
• Tissue expression is highest in brain, kidney, and testis; during fetal development, strong expression is observed in kidney, lung, small intestine, and brain. Fibroblastic cells express both isoform 1 and isoform 2, while epithelial cells solely express isoform 2.
• Mutations leading to constitutive activation of FGFR3 are associated with craniosynostosis, various forms of dwarfism, deafness, and cancer. Overexpression or aberrant activation promotes phosphorylation of STAT1, STAT5A/B, and SHP2, driving oncogenic signaling.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 700–806 within the intracellular tyrosine kinase domain of FGFR3; thus, the antibody detects the cytoplasmic portion of the receptor. For IHC-P and IF/ICC, ensure adequate permeabilization to allow access to this epitope.
• In WB, the antibody is expected to recognize FGFR3 at approximately 88 kDa. Include appropriate positive (e.g., FGFR3-expressing cell lines) and negative controls to validate specificity in your experimental system.
• For ELISA applications, the antibody can be used as capture or detection reagent; optimization of coating concentrations and pairing with other FGFR3 antibodies is recommended.
• This antibody shows cross-reactivity with mouse and rat FGFR3, making it a potential tool for rodent model studies. Confirm cross-reactivity and signal specificity in your models before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, providing this FGFR3 polyclonal antibody to support research into skeletal development, cancer biology, and receptor tyrosine kinase signaling. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for difficult-to-find targets—empowering diagnostic developers and researchers to accelerate their workflow from concept to clinic.