Anti-FGFR2 Polyclonal Antibody for WB, IF/ICC, ELISA - P21802

Core Product Specifications and Parameters

Biological Background: FGFR2 Function and Localization

• FGFR2 is a receptor tyrosine kinase that binds fibroblast growth factors, regulating cell proliferation, differentiation, migration, and apoptosis.
• Plays critical roles in embryonic development, including limb bud formation, lung morphogenesis, osteogenesis, and skin development.
• Required for normal skeleton development by regulating osteoblast differentiation, proliferation, and apoptosis.
• Ligand binding activates downstream signaling cascades via phosphorylation of FRS2 and PLCG1, leading to RAS-MAPK and AKT1 pathway activation.
• Localizes to the cell membrane, but also found in the Golgi apparatus, cytoplasmic vesicles, and can be secreted as a soluble form.
• Subject to post-translational modifications including glycosylation, disulfide bond formation, and ubiquitination-mediated down-regulation.
• Mutations in FGFR2 are associated with various skeletal and ectodermal disorders such as craniosynostosis and lacrimo-auriculo-dento-digital syndrome.
• Highly expressed in keratinocytes and osteoblasts, with alternative splicing generating isoforms like KGFR (keratinocyte growth factor receptor) that show distinct ligand specificity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic region of FGFR2, suggesting the antibody may recognize intracellular epitopes; for IF/ICC, ensure adequate cell permeabilization.
• For Western blot, a band around 92 kDa (predicted) may be observed, but the presence of isoforms and post-translational modifications could lead to additional bands; validate using positive controls.
• Cross-reactivity with mouse and rat FGFR2 is reported; when using these species, verify reactivity in the relevant tissue lysates.
• As a polyclonal antibody, lot-to-lot variability is possible; it is advisable to titrate the antibody for each application and include appropriate control antibodies.
• For sandwich ELISA applications, consider pairing with a capture antibody that targets a distinct epitope; verify assay specificity using recombinant FGFR2 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive one-stop sourcing platform for IVD raw materials. This FGFR2 polyclonal antibody supports research in developmental biology, oncology, and signal transduction, with applications in WB, IF/ICC, and ELISA. Beyond individual reagents, CamelBio offers validated antibody pairs, bulk ancillary reagents, and customized raw-material sourcing to accelerate your assay development from concept to clinic.