Anti-FGF9 Polyclonal Antibody for WB and ELISA - P31371

Core Product Specifications and Parameters

Biological Background: FGF9 Function and Localization

• FGF9 is a member of the fibroblast growth factor family, playing critical roles in embryonic development, cell proliferation, differentiation, and migration.
• It is also known as glia-activating factor (GAF) and is involved in glial cell growth and differentiation during development, as well as gliosis following brain injury.
• FGF9 supports neuronal cell differentiation and survival, and may stimulate the growth of glial tumors.
• The protein is secreted and functions as a heparin-binding growth factor, exerting its effects through receptor-mediated signaling.
• Encoded by the FGF9 gene (GeneID: 2254), the unprocessed precursor has a calculated molecular weight of 23,441 Da, consistent with the observed ~23 kDa band.
• Keywords for FGF9 include developmental protein, growth factor, mitogen, secreted, heparin-binding, and disease variants.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the N-terminal region (aa 1-100) of human FGF9, which is highly conserved across human, mouse, and rat, explaining the observed cross-reactivity.
• For Western blotting, consider optimizing antibody dilution and sample preparation, expecting a specific band at approximately 23 kDa.
• In ELISA, validate the antibody with recombinant FGF9 or tissue lysates, and note that heparin or heparan sulfate may enhance FGF9 reactivity.
• Always include positive and negative species-specific controls to confirm expected reactivity and exclude non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with premium IVD raw materials, including this anti-FGF9 polyclonal antibody for developmental and neuroscience applications. Beyond primary antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, with capabilities to source rare or custom targets. Partner with CamelBio to streamline your procurement from concept to clinical manufacturing.