Anti-FGF7/KGF Rabbit Monoclonal Antibody for WB, ELISA - P21781

Core Product Specifications and Parameters

Biological Background: FGF7/KGF Function and Localization

• Fibroblast growth factor 7 (FGF7), also known as keratinocyte growth factor (KGF) or heparin-binding growth factor 7 (HBGF-7), is encoded by the FGF7 gene and undergoes alternative splicing to produce multiple isoforms.
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• FGF7 acts as a potent mitogen specifically for epithelial cells, functioning as a paracrine mediator of epithelial cell proliferation and survival.
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• During embryonic development, FGF7 is essential for normal branching morphogenesis, contributing to the regulation of cell proliferation and differentiation in various tissues.
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• Subcellularly, FGF7 is secreted into the extracellular space, where it binds to its receptor (FGFR2b) on target epithelial cells to exert its biological effects.
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• Expressed primarily in epithelial cells, FGF7 plays a critical role in the maintenance and repair of epithelial tissues throughout the body.
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• As a heparin-binding glycoprotein, FGF7's activity is modulated by interactions with heparan sulfate proteoglycans in the extracellular matrix, and it possesses an N-terminal signal peptide for secretion.
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Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (aa 1-100) of human FGF7, which is predicted to be specific for this target.
• In western blotting, bands corresponding to the reported molecular weights (12 kDa and 23 kDa) may be observed; larger bands can result from glycosylation. Validate detection in the relevant cell or tissue lysate system.
• For ELISA applications, this antibody can be utilized in capture or detection setups; consider pairing it with a compatible antibody or using recombinant FGF7 protein as a standard for assay optimization.
• The antibody is known to cross-react with mouse and rat FGF7; for other species, verify sequence homology to ensure suitability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies. For projects targeting growth factors like FGF7/KGF in epithelial biology, CamelBio offers reliable anti-FGF7 antibodies and can assist with bulk ancillary reagents and rare target raw-material sourcing. Accelerate your assay development from concept to clinic with our one-stop procurement support.