Anti-FGF4 Rabbit Polyclonal Antibody for WB and ELISA - P08620

Core Product Specifications and Parameters

Biological Background: FGF4 Function and Localization

• FGF4 (fibroblast growth factor 4) is a secreted growth factor belonging to the fibroblast growth factor family, also known as HST, HST-1, K-FGF, and HBGF-4.
• It functions as a key regulator of embryonic development, playing essential roles in limb bud outgrowth, cardiac valve formation, and tooth bud development through induction of MSX1 and SDC1 expression in mesenchymal cells.
• FGF4 acts as a potent mitogen and differentiation factor for various cell types, signaling through FGF receptors and requiring heparin or heparan sulfate for receptor binding and activation.
• The gene for FGF4 undergoes alternative splicing, potentially producing multiple isoforms that may contribute to functional diversity.
• Subcellularly, FGF4 is a secreted protein, localized in the extracellular space where it exerts paracrine and endocrine effects.
• FGF4 is recognized as a proto-oncogene; the transforming protein KS3 corresponds to FGF4, and its overexpression has been associated with certain cancers.
• Post-translational modification and secretion are critical for its activity; it contains a signal peptide and is processed for secretion.

Experimental Guidance and Technical Tips

• The immunogen was designed as a recombinant fusion protein spanning amino acids 31-206 of human FGF4 (NP_001998.1), which corresponds to the mature secreted form, ensuring antibodies are raised against the biologically active region.
• In Western blotting, a specific band around 22 kDa is expected; consider using positive control lysates from FGF4-expressing cells or tissue known to express FGF4 during embryonic stages. Rat samples can be used given the noted cross-reactivity.
• For ELISA applications, this antibody may serve as a detection reagent; validate optimal coating and detection conditions in the relevant sample matrix, keeping in mind the heparin-binding nature of FGF4 which may influence capture.
• While only WB and ELISA are listed, investigators may explore immunohistochemistry or immunofluorescence with appropriate antigen retrieval and validation, as FGF4 is secreted and may require careful tissue fixation.
• To maintain activity, store at -20°C or below, avoid multiple freeze-thaw cycles, and use ice bag shipping as provided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive IVD raw-material supply solution, from concept to clinic. This anti-FGF4 rabbit polyclonal antibody exemplifies our offering of validated reagents for growth factor and developmental biology targets. Through CamelBio, you can efficiently source validated antibody pairs, bulk ancillary reagents, and rare-target raw materials, all backed by our commitment to quality and supply chain reliability.