Anti-FGF3 Polyclonal Antibody for WB, ELISA - P11487

Core Product Specifications and Parameters

Biological Background: FGF3 Function and Localization

• FGF3, also known as INT2 or HBGF-3, is a member of the fibroblast growth factor family and functions as a heparin-binding growth factor.
• It plays an important role in the regulation of embryonic development, including cell proliferation and differentiation, and is required for normal ear development.
• FGF3 acts as a mitogen and is involved in developmental processes, as reflected by keywords such as 'Developmental protein' and 'Differentiation'.
• The protein is secreted and contains a signal peptide, with a molecular weight of approximately 27 kDa (calculated from sequence: 26887 Da).
• Post-translational modifications include glycosylation, and it is classified as a glycoprotein.
• FGF3 is also annotated as a proto-oncogene, indicating potential involvement in oncogenic processes.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide from the N-terminal region (amino acids 1-100) of human FGF3, suggesting that the antibody may recognize the N-terminus of the protein.
• For Western blotting, consider using reducing conditions and validating the antibody in cell lines or tissues that express FGF3, such as embryonic samples.
• The antibody cross-reacts with rat FGF3, so it can be utilized in rat model systems; however, verify performance in your specific experimental context.
• In ELISA applications, optimize coating and detection conditions using a purified FGF3 protein standard if available.
• Since FGF3 is a secreted protein, consider analyzing conditioned media or cell lysates, and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic development. For FGF3-related research in embryonic development and cell differentiation, CamelBio can supply high-quality reagents and assist with rare target raw-material sourcing. Our team is dedicated to bridging the gap from concept to clinic, ensuring reliable and efficient access to the materials you need.