Anti-FGF21 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9NSA1

Core Product Specifications and Parameters

Biological Background: FGF21 Function and Localization

• Fibroblast growth factor 21 (FGF21) is a secreted protein belonging to the FGF family, which plays roles in cell growth, tissue repair, and metabolism.
• FGF21 stimulates glucose uptake in differentiated adipocytes by inducing expression of the glucose transporter SLC2A1/GLUT1, and this activity requires the presence of the co-receptor beta-klotho (KLB).
• It regulates systemic glucose homeostasis and insulin sensitivity, making it a potential therapeutic target for metabolic diseases like diabetes.
• FGF21 is secreted into the extracellular space and blood circulation, acting in an endocrine manner on target tissues.
• The protein has a molecular weight of approximately 22.3 kDa and contains a signal peptide for secretion.
• Keywords associated with FGF21 include growth factor, secreted, signal, and 3D-structure, indicating its established structural characterization.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-100 of human FGF21. This N-terminal region may be suitable for detecting both full-length and potential fragments. Consider validating with relevant sample systems.
• For WB, the predicted protein weight is 22 kDa; ensure proper sample preparation and verify band size in the relevant cell or tissue lysate.
• For IHC-P, antigen retrieval conditions may need optimization; run a pilot with positive and negative controls.
• For ELISA, use a suitable recombinant FGF21 protein as standard and validate the working concentration range.
• Cross reactivity is reported with human, mouse, and rat. Sequence homology analysis might predict reactivity with other species; always validate experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a one-stop sourcing solution for IVD raw materials, technical services, and consulting. This anti-FGF21 antibody supports studies in metabolic disorders and glucose homeostasis. As a B2B partner, CamelBio supplies validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist with rare target sourcing to advance your assay development.