Anti-FGF21 Polyclonal Antibody for WB, ELISA - Q9NSA1

Core Product Specifications and Parameters

Biological Background: FGF21 Function and Localization

• Fibroblast growth factor 21 (FGF21) is a member of the fibroblast growth factor (FGF) family, functioning as an endocrine hormone-like metabolic regulator.
• It plays a key role in systemic glucose homeostasis and insulin sensitivity, making it a critical target in metabolic disease research.
• FGF21 stimulates glucose uptake in differentiated adipocytes by inducing the expression of glucose transporter SLC2A1/GLUT1 (but not SLC2A4/GLUT4), a process dependent on the co-receptor beta-klotho (KLB).
• The protein is secreted and contains a signal peptide; the mature form (after cleavage of the signal peptide) has a predicted molecular weight of approximately 22 kDa.
• Structural studies (3D-structure) have elucidated its interaction with FGF receptors and KLB, and it has been characterized via direct protein sequencing and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature FGF21 sequence (aa 29-209), which is well-conserved between human and mouse, supporting the reported cross-reactivity; verify in your experimental system.
• For Western blot, consider testing this antibody in relevant metabolic tissues (e.g., liver, adipose) or cell lines known to express FGF21; a band near 22 kDa is expected.
• FGF21 is a secreted protein; when using ELISA, ensure appropriate sample preparation for culture supernatants or serum samples, and be aware that endogenous levels may be low under basal conditions.
• As a polyclonal antibody, inherent batch-to-batch variability is possible; validate performance in your specific application and sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, providing reliable access to high-quality antibodies like this anti-FGF21 polyclonal antibody for metabolic research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing solutions to support your diagnostic development from concept to clinic.