Anti-FGF2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09038

Core Product Specifications and Parameters

Biological Background: FGF2 Function and Localization

• Acts as a ligand for fibroblast growth factor receptors (FGFR1, FGFR2, FGFR3, FGFR4) and integrin ITGAV:ITGB3, mediating signaling cascades that regulate cell survival, proliferation, differentiation, and migration. Related references: PMID:8663044 PMID:28302677
• Functions as a potent mitogen in vitro, stimulating cell division in various cell types. Related references: PMID:1721615 PMID:3732516 PMID:3964259
• Induces angiogenesis, promoting the formation of new blood vessels, a process critical in development and tumor progression. Related references: PMID:23469107 PMID:28302677
• Mediates phosphorylation of ERK1/2, contributing to retinal lens fiber differentiation. Related references: PMID:29501879
• Is a heparin-binding growth factor, which enhances its stability and receptor interaction.
• Localizes to both the nucleus and the secreted extracellular space, suggesting intracrine and paracrine signaling roles.
• Expressed in granulosa and cumulus cells, and in hepatocellular carcinoma cells, but not in non-cancerous liver tissue, indicating potential roles in reproduction and cancer.
• Belongs to the FGF family and is also known as basic fibroblast growth factor (bFGF) and heparin-binding growth factor 2 (HBGF-2).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 143-288) of human FGF2, which may enable detection of both full-length and certain truncated forms.
• For Western blotting, the theoretical molecular weight is approximately 31 kDa; however, post-translational modifications may cause band shifts. Validate detection in appropriate positive controls.
• In immunohistochemistry, consider optimizing antigen retrieval (e.g., heat-mediated citrate buffer) and include isotype controls to confirm specificity.
• For immunofluorescence, permeabilization is required for nuclear staining; test fixation conditions (e.g., 4% PFA) to balance signal and morphology.
• ELISA performance can be assessed by coating the antigen directly or using a sandwich format with a matched antibody pair.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For FGF2-related research in angiogenesis, oncology, and developmental biology, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our sourcing network also enables procurement of rare-target raw materials tailored to your assay development needs.