Anti-FGF2 Rabbit Polyclonal Antibody for WB, ELISA - P09038

Core Product Specifications and Parameters

Biological Background: FGF2 Function and Localization

• FGF2, also known as basic fibroblast growth factor (bFGF) or heparin-binding growth factor 2 (HBGF-2), is encoded by the FGF2 (FGFB) gene.
• Acts as a high-affinity ligand for fibroblast growth factor receptors FGFR1, FGFR2, FGFR3, and FGFR4, and also binds integrin ITGAV:ITGB3 as a co-receptor required for signaling.
  Related references: PMID:8663044 PMID:28302677
• Functions as a potent mitogen, regulating cell survival, proliferation, differentiation, and migration in various cell types.
  Related references: PMID:1721615 PMID:3732516 PMID:3964259
• Promotes angiogenesis, the formation of new blood vessels from pre-existing vasculature.
  Related references: PMID:23469107 PMID:28302677
• Mediates phosphorylation of ERK1/2 (MAPK1/3) and thereby drives retinal lens fiber cell differentiation.
  Related references: PMID:29501879
• Subcellular localization includes secretion into the extracellular space and nuclear translocation; FGF2 contains a heparin-binding domain and can be released through unconventional protein secretion.
• Expressed in granulosa and cumulus cells, as well as in hepatocellular carcinoma cells, but not in normal liver tissue, indicating a potential role in tumorigenesis.
• Post-translational modifications include phosphorylation, methylation, and ubiquitination; alternative initiation produces isoforms with different N-termini.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100-200 of human FGF2. Given the reported cross-reactivity with mouse and rat, sequence alignment in this region is likely conserved; however, users should verify reactivity in their specific experimental systems.
• For Western blotting, a band around 31 kDa is expected under reducing conditions. Due to alternative translation initiation, multiple isoforms ranging from 18-34 kDa may be observed depending on the cell type and lysis conditions.
• Polyclonal antibodies may exhibit lot-to-lot variability. It is advisable to titrate the antibody and include appropriate positive and negative controls in each assay.
• For ELISA, the antibody can be used as a detection reagent; optimal working concentration should be determined empirically. Consider pairing with a monoclonal capture antibody for improved specificity if needed.
• Store and handle according to the manufacturer's instructions; avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD raw materials, providing validated antibodies, antigen pairs, and ancillary reagents to accelerate diagnostic assay development. This anti-FGF2 polyclonal antibody, targeting a key growth factor in cell proliferation and angiogenesis, supports research and assay design in oncology and regenerative medicine. Our supply capabilities include bulk monoclonal and polyclonal antibodies, and we expertly source difficult-to-find targets to meet your project needs.