Anti-FGF2 Rabbit Monoclonal Antibody for WB, ELISA - P09038

Core Product Specifications and Parameters

Biological Background: FGF2 Function and Localization

• The FGF2 gene encodes fibroblast growth factor 2, also known as basic fibroblast growth factor (bFGF) and heparin-binding growth factor 2 (HBGF-2).
• FGF2 acts as a ligand for FGFR1, FGFR2, FGFR3, and FGFR4, and also functions as an integrin ligand, regulating cell survival, division, differentiation, and migration. Related references: PMID:8663044, PMID:28302677
• It functions as a potent mitogen in vitro and can induce angiogenesis. Related references: PMID:1721615, PMID:3732516, PMID:3964259, PMID:23469107, PMID:28302677
• FGF2 mediates phosphorylation of ERK1/2, promoting retinal lens fiber differentiation. Related references: PMID:29501879
• The protein is secreted and also localizes to the nucleus.
• Tissue expression includes granulosa and cumulus cells, and it is found in hepatocellular carcinoma cells but not in non-cancerous liver tissue.
• Post-translational modifications include isopeptide bond formation, methylation, phosphorylation, and ubiquitin conjugation.
• Alternative initiation produces multiple isoforms with molecular weights of 17, 21, 22, and 30 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans residues 100–200 of human FGF2, a region likely to recognize conserved epitopes present in multiple isoforms (17–30 kDa).
• Based on high sequence homology, cross-reactivity with mouse and rat FGF2 is anticipated; validate in the relevant model system.
• For Western blot, consider sample preparation that includes both nuclear and secreted fractions to capture all localizations.
• In ELISA, optimize antibody concentration and detection system using purified recombinant FGF2 as a reference.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering diagnostic manufacturers streamlined access to validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents. For FGF2-related oncology and angiogenesis research, we provide consistent supply and technical support to accelerate your assay development.