Anti-FGF13 Rabbit Polyclonal Antibody for WB - Q92913

Core Product Specifications and Parameters

Biological Background: FGF13 Function and Localization

• FGF13, also known as fibroblast growth factor homologous factor 2 (FHF-2), is a member of the fibroblast growth factor homologous factor (FHF) subfamily, distinct from canonical FGFs.
• It functions as a microtubule-binding protein that directly interacts with tubulin, promoting microtubule polymerization and stabilization, which is crucial for neuronal polarization and migration.
• FGF13 regulates the transport and function of voltage-gated sodium channels, impacting neuronal excitability. Related references: PMID:15282281, PMID:33245860, PMID:36696443
• It undergoes alternative splicing, producing multiple isoforms that may display distinct subcellular localizations including the nucleus, cytoplasm, and various cell projections such as filopodia and growth cones.
• The protein is ubiquitously expressed, with predominant expression in the nervous system, where it plays roles in axon refinement and the development of inhibitory GABAergic synapses.
• Post-translational modifications include phosphorylation, and mutations in FGF13 are associated with diseases such as epilepsy and X-linked intellectual disability (keywords: Disease variant, Epilepsy, Intellectual disability).
• Additional notes: It localizes to the sarcolemma and dendrites, and is involved in MAPK signaling (by similarity).

Experimental Guidance and Technical Tips

• Since the immunogen comprises the N-terminal 245 amino acids of human FGF13, the antibody may recognize multiple isoforms and is suitable for detecting total FGF13 levels in denaturing Western blot (WB). Consider testing at 1:500–1:2000 dilution and validate in your sample system.
• The predicted molecular weight based on the full-length sequence is ~28 kDa, but alternative splicing may produce isoforms of varying sizes; confirm band patterns with appropriate controls.
• For ELISA applications, the recombinant protein immunogen facilitates direct binding assays. Titrate the antibody and use a matched secondary antibody for signal detection.
• Cross-reactivity with mouse and rat FGF13 is reported (high sequence homology), but verify cross-reactivity in the target species by sequence alignment and pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to IVD raw materials across the entire development pipeline. This anti-FGF13 polyclonal antibody supports neuroscience research on neuronal development and ion channel function. For bulk antibody orders, custom production, or complementary reagent sourcing, contact CamelBio to bridge your supply needs.