Anti-FGF1 Rabbit PolymAb® for WB, IF/ICC, IHC-P, ELISA - P05230

Core Product Specifications and Parameters

Biological Background: FGF1 Function and Localization

• Fibroblast growth factor 1 (FGF1), also known as acidic FGF (aFGF), endothelial cell growth factor (ECGF), and heparin-binding growth factor 1 (HBGF-1), is a member of the fibroblast growth factor family.
• FGF1 acts as a potent mitogen and plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation, and cell migration.
• It functions as a ligand for FGFR1 and integrins (ITGAV:ITGB3), triggering downstream signaling. FGF1 induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2, and AKT1. Related references: PMID:18441324, PMID:20422052
• FGF1 can induce angiogenesis, contributing to new blood vessel formation. Related references: PMID:23469107
• Subcellularly, FGF1 is found in the cytoplasm, cell cortex, cytosol, and nucleus, and it is also secreted.
• Predominantly expressed in the kidney and brain, with much lower levels detected in the heart and skeletal muscle.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity and interactions.
• Keywords: Angiogenesis, differentiation, developmental protein, growth factor, heparin-binding, mitogen, nucleus, secreted, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 56-155 of human FGF1, covering the mature protein sequence; consider using recombinant full-length or mature FGF1 as a positive control.
• For Western blotting (WB), the predicted molecular weight is ~17 kDa; optimize loading and transfer conditions for low-molecular-weight proteins.
• FGF1 is heparin-binding; in immunoassays, the inclusion of heparin or low-ionic-strength buffers may influence antibody-antigen interactions and should be assessed.
• When performing immunohistochemistry (IHC-P) or immunofluorescence (IF/ICC), validate staining in high-expression tissues such as kidney or brain.
• For ELISA, titrate the antibody against recombinant FGF1 standards and confirm linearity and sensitivity in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and monoclonal/polyclonal antibodies like this FGF1 PolymAb®. Target areas such as oncology, cardiovascular disease, and neurobiology can benefit from our bulk ancillary reagents and rare target sourcing support. We streamline procurement from concept to clinic, helping you accelerate assay development with reliable, one-stop access to critical raw materials.