Anti-FGF1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P05230

Core Product Specifications and Parameters

Biological Background: FGF1 Function and Localization

• FGF1 is a fibroblast growth factor family member, also known as acidic FGF (aFGF), endothelial cell growth factor (ECGF), and heparin-binding growth factor 1 (HBGF-1).
• It regulates cell survival, division, angiogenesis, differentiation, and migration, acting as a potent mitogen in vitro.
• FGF1 binds to FGFR1 with heparin, leading to receptor dimerization, autophosphorylation, and activation of downstream signaling cascades.
• It induces phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2, and AKT1. Related references: PMID:18441324 PMID:20422052
• FGF1 can induce angiogenesis, a process crucial for tissue repair and tumor growth. Related references: PMID:23469107
• FGF1 also binds to integrin ITGAV:ITGB3, forming a ternary complex with integrin and FGFR1, essential for signaling.
• Subcellularly, FGF1 localizes to the secreted space, cytoplasm, cell cortex, cytosol, and nucleus.
• It is predominantly expressed in kidney and brain, with lower levels in heart and skeletal muscle; post-translational modifications include acetylation and phosphorylation, and alternative splicing yields multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 16-155 of human FGF1, covering the mature protein, which may confer broad reactivity with the native form.
• For WB, a band at approximately 17 kDa is expected; however, post-translational modifications may shift the observed molecular weight.
• In IHC-P and IF/ICC, consider using appropriate fixation and antigen retrieval methods; validate in tissues known to express FGF1 such as kidney or brain.
• ELISA performance may vary; consider sandwich ELISA development with a capture/detection pair.
• Cross-reactivity with mouse and rat FGF1 is indicated; verify specificity in the relevant species context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For FGF1-related research, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and support for rare target raw-material procurement. Contact us to streamline your IVD development with reliable, high-quality reagents.