Anti-FGF1 Monoclonal Antibody for WB, IF/ICC, ELISA - P05230

Core Product Specifications and Parameters

Biological Background: FGF1 Function and Localization

• FGF1, also known as acidic fibroblast growth factor (aFGF), endothelial cell growth factor (ECGF), and heparin-binding growth factor 1 (HBGF-1), is a member of the fibroblast growth factor family that acts as a potent mitogen and regulates cell survival, division, angiogenesis, differentiation, and migration. Related references: PMID:18441324 PMID:20422052
• It functions as a ligand for fibroblast growth factor receptor 1 (FGFR1) and integrin ITGAV:ITGB3, requiring heparin for receptor binding and activation. Ligand binding induces FGFR1 dimerization and autophosphorylation, initiating downstream signaling cascades including MAPK/ERK and AKT1 pathways. Related references: PMID:18441324 PMID:20422052
• FGF1 can induce angiogenesis, a process critical for vascular development and tissue repair. Related references: PMID:23469107
• The protein is predominantly expressed in kidney and brain, with lower levels in heart and skeletal muscle, reflecting its tissue-specific roles.
• Subcellular localization includes secretion, cytoplasm, cell cortex, cytosol, and nucleus, indicating diverse functional compartments.
• Post-translational modifications of FGF1 include acetylation and phosphorylation, which may regulate its activity and interactions.
• FGF1 is involved in developmental processes and is classified as a heparin-binding growth factor, emphasizing its role in cell signaling and extracellular matrix interactions.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight of FGF1 is 17 kDa; however, post-translational modifications may result in observed bands at slightly different sizes. Validate antibody specificity using positive and negative controls.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to detect FGF1 in its cytoplasmic, nuclear, or secreted states, depending on the cellular context.
• For ELISA applications, use the antibody as capture or detection reagent after confirming reactivity with recombinant FGF1 protein and biological samples.
• Cross-reactivity with mouse and rat has been documented; researchers working with these species should validate the antibody in the relevant sample systems and consider potential differences in protein expression levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with efficient access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this anti-FGF1 reagent, and bulk ancillary reagents. With expertise in signaling pathways and developmental biology, we support your workflow from target selection to clinical application. Contact us to explore tailored sourcing solutions for your next project.