Anti-FGD1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P98174

Core Product Specifications and Parameters

Biological Background: FGD1 Function and Localization

• FGD1 (FYVE, RhoGEF and PH domain-containing protein 1), also known as faciogenital dysplasia 1 protein or ZFYVE3, is a guanine nucleotide exchange factor (GEF) for the small GTPase CDC42.
• By catalyzing the exchange of GDP for GTP, it activates CDC42, thereby regulating the actin cytoskeleton and cell shape, as well as formation of cell projections such as lamellipodia and ruffles.
• The protein contains FYVE, RhoGEF (DH), and PH domains, with the FYVE domain binding phosphatidylinositol 3-phosphate and the DH-PH module mediating nucleotide exchange activity.
• FGD1 localizes to the cytoplasm and associates with the cytoskeleton, particularly at lamellipodia and membrane ruffles, consistent with its role in cytoskeletal remodeling.
• It is expressed in fetal heart, brain, lung, kidney, and placenta, with lower levels in adult tissues including heart, brain, lung, pancreas, and skeletal muscle.
• Ubiquitously expressed and phosphorylated, FGD1 is classified under keywords including Cell projection, Cytoplasm, Cytoskeleton, and Guanine-nucleotide releasing factor.
• Mutations in FGD1 cause faciogenital dysplasia (Aarskog-Scott syndrome), an X-linked disorder characterized by craniofacial, skeletal, and urogenital anomalies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 700-800 of human FGD1; align this region with mouse and rat orthologs to confirm epitope conservation and expected cross-reactivity.
• For Western blot, the predicted molecular weight is approximately 107 kDa; optimize running and transfer conditions for large proteins, and validate the band specificity using appropriate positive controls.
• In IHC-P and IF/ICC, consider that FGD1 localizes to the cytoplasm and cytoskeleton; permeabilization and fixation protocols should be adjusted accordingly, and signal may be enriched at lamellipodia and ruffles.
• ELISA: determine optimal coating concentration of the antibody or antigen for indirect or sandwich formats, and include relevant validation with recombinant protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials and technical services. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies such as anti-FGD1, and bulk ancillary reagents for actin cytoskeleton and CDC42 signaling studies. Contact CamelBio for rare target sourcing and end-to-end support from concept to clinical application.