Anti-FFAR4 Rabbit Polyclonal Antibody for WB, ELISA - Q5NUL3

Core Product Specifications and Parameters

Biological Background: FFAR4 Function and Localization

• FFAR4 (Free fatty acid receptor 4), also known as GPR120, GPR129, O3FAR1, and by other aliases, is a G protein-coupled receptor for long-chain fatty acids.
• It signals via phosphoinositidase C-linked G proteins GNAQ/GNA11 (Gq/G11) and beta-arrestin pathways, modulating intracellular calcium, cAMP, and MAP kinases. Related references: PMID:22282525, PMID:22343897, PMID:24742677
• Upon ligand binding, FFAR4 associates with beta-arrestin ARRB2, which couples the receptor to specific downstream signaling and mediates receptor endocytosis. Related references: PMID:22282525, PMID:24817122
• FFAR4 mediates the anti-inflammatory effects of omega-3 PUFAs by inhibiting NLRP3 inflammasome activation through ARRB2 association. Related references: PMID:23809162
• It is localized in the cell membrane, endosome and lysosome membranes, and primary cilium membrane of preadipocytes.
• Expressed in adipose tissue, pancreatic islets (alpha and delta cells), lung, brain, and intestinal neuroendocrine cells, with a role in hormone secretion and appetite regulation.
• Post-translational modifications include glycosylation and phosphorylation; the receptor has a molecular weight of approximately 40 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 333–377 of human FFAR4, a C-terminal region. This design may enable detection of multiple isoforms.
• For western blotting, a band around 40 kDa is expected. Validate using known positive tissues such as adipose tissue or pancreatic islet cell lines.
• In ELISA, consider using purified recombinant FFAR4 protein as a standard to optimize antibody concentration.
• Cross-reactivity with mouse and rat FFAR4 is indicated; verify in the relevant species model as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-FFAR4 pAb, and ancillary reagents. For metabolic receptor research and assay development, we source high-quality antibodies, proteins, and rare target materials to streamline your workflow from concept to clinic.