Anti-Fetuin A/AHSG Polyclonal Antibody for WB, ELISA - P02765

Core Product Specifications and Parameters

Biological Background: Fetuin A/AHSG Function and Localization

• Alpha-2-HS-glycoprotein (AHSG), also known as Fetuin-A, is a secreted plasma protein belonging to the fetuin family.
• Fetuin-A promotes endocytosis and exhibits opsonic properties, enhancing the clearance of cellular debris and pathogens.
• It influences the mineral phase of bone and shows affinity for calcium and barium ions, playing a role in mineral homeostasis.
• The protein is primarily synthesized in the liver and secreted into plasma; it is also selectively concentrated in bone matrix and found at high levels in dentin.
• AHSG is a glycoprotein with disulfide bonds and phosphorylated residues, and it contains internal repeats characteristic of the fetuin family.
• The mature protein has a molecular weight of approximately 39-40 kDa after signal peptide cleavage.

Experimental Guidance and Technical Tips

• Typical working dilutions for Western blotting range from 1:500 to 1:5000; however, optimal dilution should be determined by the end user.
• For ELISA, consider pairing with a monoclonal antibody for sandwich ELISA to detect recombinant or native Fetuin-A.
• Due to glycosylation, the observed molecular weight in Western blot may appear higher than the predicted 39 kDa.
• The immunogen spans amino acids 19-300, covering the majority of the mature protein; therefore, the antibody is likely to recognize both native and denatured Fetuin-A.
• Validate antibody specificity in the relevant sample matrix, such as human serum or plasma for circulatory Fetuin-A.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes comprehensive one-stop access to IVD raw materials, technical services, and consulting. For projects targeting liver function, bone metabolism, or inflammation where Fetuin A/AHSG is a key analyte, we supply validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing. Our integrated supply chain empowers your development from early research to clinical validation.