Anti-Ferrochelatase (FECH) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P22830

Core Product Specifications and Parameters

Biological Background: Ferrochelatase Function and Localization

• Ferrochelatase (FECH), also known as heme synthase or protoheme ferro-lyase, is a mitochondrial enzyme that catalyzes the terminal step in heme biosynthesis: the insertion of ferrous iron into protoporphyrin IX to form protoheme.
• It is an essential member of the heme biosynthetic pathway, operating at the matrix side of the mitochondrial inner membrane.
• FECH contains a [2Fe-2S] cluster critical for its enzymatic activity, and is subject to acetylation and alternative splicing, generating multiple isoforms.
• Mutations in the FECH gene are associated with erythropoietic protoporphyria, an inherited disorder of porphyrin metabolism.
• The protein is widely studied as a reference proteome component, with its three-dimensional structure resolved and proteomic profiles established across tissues.

Experimental Guidance and Technical Tips

• For Western blotting, a major band around 48 kDa is expected for FECH; consider optimizing protein extraction and antibody dilution for clear detection.
• The antibody's suitability for IHC-P suggests its potential for tissue localization studies; antigen retrieval conditions and antibody concentration may require optimization for individual tissue types.
• ELISA can be employed to assess antibody titer using the recombinant immunogen or to quantify FECH in sample matrices, but validation in the relevant sample system is advised.
• Given cross-reactivity with mouse and rat, this antibody is a candidate for comparative studies across species; however, signal specificity should be confirmed in each model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-FECH antibody exemplifies our capability to supply critical tools for heme biosynthesis and mitochondrial disease research. Contact us to explore custom sourcing for rare targets or to scale up your supply chain seamlessly.