Anti-Ferritin Heavy Chain Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P02794

Core Product Specifications and Parameters

Biological Background: Ferritin Heavy Chain Function and Localization

• Ferritin heavy chain (FTH1, also known as FTH, FTHL6, cell proliferation-inducing gene 15 protein) is a key iron storage protein belonging to the ferritin family. It assembles into a 24-subunit shell to store iron in a soluble, non-toxic form.
• The protein possesses ferroxidase activity, catalyzing the oxidation of Fe²⁺ to Fe³⁺ and thereby facilitating iron deposition as ferric hydroxides within the ferritin cage (PubMed:9003196).
  Related references: PMID:9003196
• Beyond storage, ferritin heavy chain mediates iron delivery to cells, including specialized uptake in developing kidney capsule cells, and participates in intracellular iron trafficking.
• Its lysosomal degradation is regulated by the cargo receptor NCOA4, which directs ferritin to autophagosomes for iron release—a process critical for cellular iron homeostasis (PubMed:24695223, PubMed:26436293).
  Related references: PMID:24695223 PMID:26436293
• Subcellular localization includes the cytoplasm, lysosomes, and cytoplasmic vesicles such as autophagosomes, reflecting its dynamic role in iron storage and recycling.
• The protein is expressed in the liver, a major iron storage organ, and its expression can be regulated by iron levels and oxidative stress.
• Post-translational modifications including acetylation and phosphorylation, along with its oxidoreductase activity, fine-tune ferritin function and stability; the molecule is also implicated in neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human ferritin heavy chain (aa 1–183), making this polyclonal antibody suitable for detecting native protein conformations across multiple applications.
• For Western blotting, the predicted molecular weight is approximately 21 kDa; consider using a 12–15% SDS-PAGE gel for optimal separation.
• In immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) should be optimized for each tissue type; validate with appropriate positive and negative controls.
• When using immunofluorescence (IF/ICC), fixation and permeabilization methods may influence signal; test methanol or paraformaldehyde fixation with Triton X-100 permeabilization.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with a monoclonal antibody against a non-overlapping epitope may improve assay performance—validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials and technical services. This anti-Ferritin Heavy Chain polyclonal antibody supports research into iron metabolism, neurodegeneration, and related disorders. Our supply chain spans validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your development from concept to clinic.