Anti-Ferritin Heavy Chain Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P02794

Core Product Specifications and Parameters

Biological Background: Ferritin Heavy Chain Function and Localization

• Ferritin heavy chain stores iron in a soluble, non-toxic form and exhibits ferroxidase activity, oxidizing ferrous iron to ferric hydroxides for safe deposition. Related references: PMID:9003196
• It mediates iron delivery to cells and plays a role in iron uptake in capsule cells of the developing kidney (By similarity).
• Iron release is regulated via NCOA4-mediated delivery to lysosomes for autophagic degradation. Related references: PMID:24695223 PMID:26436293
• Primarily localized in the cytoplasm, but also found in lysosomes, autophagosomes, and cytoplasmic vesicles.
• Post-translational modifications include acetylation and phosphorylation; linked to neurodegeneration.
• Expressed in the liver; part of the ferritin family with a predicted molecular weight of ~21 kDa.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide derived from human Ferritin Heavy Chain. Cross-reactivity with mouse and rat suggests conserved epitope recognition; consider sequence alignment to verify.
• For Western blotting, the target protein runs at ~21 kDa. Use liver lysates as a positive control given its expression profile.
• In immunofluorescence and immunohistochemistry, permeabilization steps may be required due to the cytoplasmic localization of the protein.
• For ELISA applications, consider pairing with a detection antibody against a distant epitope for sandwich assay development. Validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, from validated monoclonal antibodies like this anti-FTH1 antibody to optimized antibody pairs and bulk ancillary reagents. Whether your project involves iron metabolism, autophagy, or neurodegeneration studies, our supply chain supports your development with rare target sourcing and comprehensive procurement solutions. We help bridge the gap from concept to clinic with tailored material supply.