Anti-Ferritin Heavy Chain Monoclonal Antibody for WB, IF-P, ELISA - P02794

Core Product Specifications and Parameters

Biological Background: Ferritin Heavy Chain Function and Localization

• Ferritin heavy chain (FTH1), also known as cell proliferation-inducing gene 15 protein and encoded by the FTH1 gene, is the heavy subunit of ferritin, the major intracellular iron storage protein complex.
• It maintains iron homeostasis by storing iron in a soluble, non-toxic, and bioavailable form, and exhibits ferroxidase activity, converting toxic Fe²⁺ to Fe³⁺ for safe storage. Related references: PMID:9003196
• The protein also mediates iron uptake and delivery to cells, with delivery to lysosomes for autophagic degradation and iron release facilitated by the cargo receptor NCOA4. Related references: PMID:24695223 PMID:26436293
• Subcellular localization includes cytoplasm, lysosomes, and autophagosomes, consistent with its roles in iron trafficking and recycling.
• FTH1 is expressed in the liver, a key site for iron storage and metabolism, and is subject to post-translational modifications such as acetylation and phosphorylation, as indicated by UniProt keywords.
• Keywords: oxidoreductase, metal-binding, iron storage, neurodegeneration, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 1-100 of human Ferritin heavy chain, targeting the N-terminal region; consider this when performing epitope mapping or competition assays.
• Verified applications include Western blot (predicted band ~21 kDa), immunofluorescence-paraffin, and ELISA. Optimize primary antibody dilution and incubation conditions for your specific sample type and protocol.
• Cross-reactivity with mouse and rat has been reported; validate species-specific reactivity in your experimental system, and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with efficient access to a broad portfolio of IVD raw materials, including validated antibodies for iron metabolism and related research targets like Ferritin Heavy Chain. Our integrated supply chain supports your projects from early development through clinical application, offering monoclonal antibodies, antibody pairs, and technical consultation to streamline your workflow. Partner with CamelBio for reliable sourcing and accelerate your assay development with confidence.