Anti-Ferredoxin Reductase Rabbit Monoclonal Antibody for WB - P22570

Core Product Specifications and Parameters

Biological Background: Ferredoxin Reductase Function and Localization

• Ferredoxin Reductase, officially NADPH:adrenodoxin oxidoreductase (mitochondrial), is encoded by the FDXR gene and is also known as ferredoxin--NADP(+) reductase.
• Serves as the first electron transfer protein in all mitochondrial P450 systems, including cholesterol side-chain cleavage in steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.
• Acts as a ferredoxin--NADP(+) reductase essential for coenzyme Q biosynthesis; together with FDX2, transfers electrons required for the hydroxylation reaction performed by COQ6. Related references: PMID:38425362
• Localizes to the mitochondrion inner membrane, consistent with its role in mitochondrial electron transport.
• Belongs to the flavoprotein family and utilizes FAD and NADP cofactors, functioning as an oxidoreductase in electron transport.
• Involved in lipid and steroid metabolism, with genetic variants associated with diseases like deafness, neurodegeneration, and neuropathy, as highlighted by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 142–491 of human Ferredoxin Reductase, covering a large portion of the mature protein; consider this when designing epitope mapping or competitive assays.
• For western blot (WB), the predicted molecular weight is approximately 54 kDa; validate in your sample system as mobility may vary due to post-translational modifications or processing.
• In immunofluorescence (IF/ICC and IF-P) and immunohistochemistry (IHC-P), expect mitochondrial staining; include appropriate organelle markers to confirm localization.
• For ELISA applications, the antibody’s performance may depend on the plate coating and detection format; we recommend optimizing concentrations and incubation conditions for your specific assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, enabling streamlined access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials. For mitochondria-related targets such as Ferredoxin Reductase, essential in steroid metabolism and P450 systems research, we offer comprehensive procurement solutions. Let CamelBio bridge your development needs from concept to clinic with reliable supply and expert support.