Anti-Ferredoxin Reductase Polyclonal Antibody for WB, IF-P, ELISA - P22570

Core Product Specifications and Parameters

Biological Background: Ferredoxin Reductase Function and Localization

• Ferredoxin reductase (FDXR), also known as adrenodoxin reductase (ADR/ADXR), is a mitochondrial flavoprotein that serves as the first electron transfer protein in all mitochondrial cytochrome P450 systems.
• It plays a critical role in cholesterol side-chain cleavage for steroidogenesis in steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, vitamin D metabolism in the kidney, and sterol C-27 hydroxylation in the liver (By similarity).
• Together with ferredoxin FDX2, FDXR transfers electrons to COQ6 for the hydroxylation step essential in coenzyme Q biosynthesis.
  Related references:
  PMID:38425362
• FDXR is localized to the mitochondrial inner membrane, consistent with its role in electron transport chains.
• The protein is associated with several key biological processes, including cholesterol and steroid metabolism, lipid metabolism, and electron transport, as indicated by UniProt keywords.
• Additionally, FDXR has been linked to disease variants causing deafness, neurodegeneration, and neuropathy, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 54 kDa is expected. Consider optimizing blocking and antibody dilution for your specific sample type.
• In immunofluorescence (IF-P), the antibody is expected to stain mitochondria; co-staining with mitochondrial markers can confirm localization.
• The immunogen corresponds to amino acids 142-491, covering a large portion of the protein, which may provide robust detection across species.
• Validate the antibody in the relevant sample system, such as steroidogenic tissues or cell lines, to confirm specificity.

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CamelBio offers a comprehensive platform for sourcing high-quality IVD raw materials, including validated primary antibodies like this anti-Ferredoxin Reductase polyclonal antibody. Whether you require bulk supplies, customized reagents, or rare target antibodies for research in mitochondrial metabolism and steroidogenesis, CamelBio streamlines procurement from concept to clinic. Partner with us to accelerate your diagnostic development with reliable, one-stop material solutions.