Anti-FERMT2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q96AC1

Core Product Specifications and Parameters

Biological Background: Fermitin family homolog 2 Function and Localization

• Fermitin family homolog 2 (FERMT2), also known as Kindlin-2, MIG-2, and PLEKHC1, is a scaffolding protein encoded by the FERMT2 gene in humans.
• Core function: FERMT2 enhances integrin activation mediated by TLN1/TLN2, though it activates integrins weakly by itself. It binds to phosphoinositide-rich membranes and is essential for integrin-mediated cell adhesion, cell spreading, and focal adhesion assembly.
• It participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton, orchestrating actin assembly and cell shape modulation, and recruits FBLIM1 to focal adhesions.
• FERMT2 plays a role in TGFB1 and integrin signaling pathways, stabilizes active CTNNB1, and is involved in the regulation of Wnt signaling transcription mediated by CTNNB1/TCF7L2.
• Subcellular localization: FERMT2 is found in the cytoplasm, at cell cortex, along stress fibers, at focal adhesions, on lamellipodium membrane, in the nucleus, and at the I band of sarcomeres in myofibrils. It also localizes to the cell surface.
• Tissue distribution: Ubiquitously expressed, with notable presence in numerous tumor tissues, indicating a broad functional role.
• As a phosphoprotein and alternative splicing product, FERMT2 undergoes post-translational modifications that may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 380–550 of human FERMT2, a region that may contain conserved motifs across species, consistent with the reported cross-reactivity with mouse and rat.
• For Western blotting, a band near the predicted molecular weight of 78 kDa is expected; consider using positive control lysates from human, mouse, or rat cell lines expressing FERMT2.
• In immunohistochemistry (paraffin-embedded sections), validate staining in tissues known to express FERMT2 (e.g., tumor tissues or tissues rich in focal adhesions), and optimize antigen retrieval conditions.
• For ELISA, this polyclonal antibody can be explored as a capture or detection reagent; however, we recommend performing a titration experiment and validating specificity using recombinant FERMT2 protein or verified positive samples.
• As with any polyclonal antibody, lot-to-lot variability may occur; it is advisable to test each new batch in the intended application with appropriate reference controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, from concept to clinic. This anti-FERMT2 polyclonal antibody supports research into integrin signaling, cell adhesion, and Wnt pathway biology, and is backed by our extensive supply network. Whether you need validated antibody pairs, optimized monoclonal or polyclonal antibodies, bulk ancillary reagents, or sourcing of rare-target raw materials, CamelBio provides tailored B2B solutions to meet your diagnostic development needs.