Anti-FER1L5 Polyclonal Antibody for WB, IF-P, ELISA - A0AVI2

Core Product Specifications and Parameters

Biological Background: FER1L5 Function and Localization

• Fer-1-like protein 5 (FER1L5) is a calcium-binding protein required for the Ca²⁺-activated fusion of the acrosomal membrane with the plasma membrane during the sperm acrosome reaction.
• It may also function in skeletal muscle cell development by facilitating effective myoblast fusion and membrane repair.
• Predominantly localizes to the cell membrane and cytoplasmic vesicle membranes.
• Belongs to a protein family characterized by multiple internal repeats and transmembrane domains.
• Activity is dependent on calcium binding, with the protein containing metal-binding sites.
• The FER1L5 gene undergoes alternative splicing, potentially generating multiple functionally distinct isoforms.
• Proteomics identification confirms expression in tissues where acrosome reaction and muscle development occur.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1350‑1670 of human FER1L5 (NP_001280012.1), covering a C‑terminal region that may provide isoform‑specific detection.
• For Western blotting, a band near 238 kDa is expected; consider using a low‑percentage SDS‑PAGE gel (e.g., 6%) to improve resolution of this high‑molecular‑weight protein.
• In immunofluorescence (IF‑P), punctate membrane and vesicular patterns may be observed; validate staining in spermatozoa or muscle cell lines known to express FER1L5.
• Cross‑reactivity with mouse and rat orthologs has been reported; verify performance in your model system before use.
• For ELISA, the recombinant immunogen can serve as a positive control; optimize coating concentrations and detergent conditions to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research teams with high-quality reagents like this anti‑FER1L5 polyclonal antibody. Complementing your male fertility and muscle biology research, we offer one‑stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. From early‑stage assay development to clinical translation, CamelBio streamlines procurement so you can focus on accelerating discovery.