Anti-FER Polyclonal Antibody for WB and ELISA - P16591

Core Product Specifications and Parameters

Biological Background: FER Function and Localization

• FER (Tyrosine-protein kinase Fer) is an intracellular non-receptor tyrosine kinase belonging to the FES/FPS kinase family; it is also known as TYK3, p94-Fer, and PPP1R74. Isoform 1 is ubiquitously expressed, while isoform 3 is detected in testis and certain carcinomas.
• It acts downstream of growth factor receptors (e.g., EGFR, KIT, PDGFRA/B), mediating signal transduction to promote cell proliferation, differentiation, and survival.
• FER phosphorylates multiple substrates including CTTN, FAK, GAB1, PECAM1, and STAT3, thereby regulating the actin cytoskeleton, microtubule dynamics, lamellipodia formation, and cell adhesion/migration.
• It plays a critical role in leukocyte chemotaxis and transendothelial migration upon LPS stimulation, as well as in mast cell degranulation via the FCER1 pathway.
• In the nervous system, FER contributes to synapse organization, synaptic vesicle trafficking, and neuron-neuron transmission; it is also implicated in neuronal cell death after brain injury.
• FER localizes to the cytoplasm, cytoskeleton, cell cortex, membrane, nucleus, and cell junctions, reflecting its involvement in diverse signaling complexes.
• Key post-translational modifications include phosphorylation, and it contains Src homology 2 (SH2) and protein kinase domains.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 200-450 of human FER, a region encompassing the SH2 domain and part of the catalytic kinase domain; this design may ensure detection of both full-length and truncated FER isoforms by Western blot.
• For Western blotting, a band at approximately 95 kDa is expected; however, actual observed molecular weight can vary depending on cell type and post-translational modifications.
• The antibody has been validated for WB and ELISA; it demonstrates cross-reactivity with mouse and rat FER, enabling its use across multiple model organisms.
• When performing ELISA, proper antigen coating and blocking conditions should be optimized experimentally to achieve the best signal-to-noise ratio.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; it is advisable to include appropriate positive and negative controls in each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supplying high-quality antibodies, antigens, and ancillary reagents to diagnostic manufacturers worldwide. For research on non-receptor tyrosine kinases like FER, CamelBio facilitates seamless procurement of validated polyclonal and monoclonal antibodies, helping to streamline assay development and validation.