Anti-FEN-1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P39748

Core Product Specifications and Parameters

Biological Background: FEN-1 Function and Localization

• Encodes Flap endonuclease 1 (FEN-1), also known as DNase IV, Flap structure-specific endonuclease 1, and Maturation factor 1 (MF1).
• Functions as a structure-specific nuclease with both 5'-flap endonuclease and 5'-3' exonuclease activities, essential for DNA replication and repair.
• During DNA replication, cleaves the 5'-overhanging flap structure generated by displacement synthesis, allowing ligation of Okazaki fragments.
• Participates in long patch base excision repair (LP-BER) by cleaving within apurinic/apyrimidinic (AP) site-terminated flaps.
• Acts as a genome stabilization factor, preventing DNA secondary structures that could lead to duplications and deletions.
• Also exhibits 5'-3' exonuclease activity on nicked/gapped dsDNA, RNase H activity, and is involved in rDNA and mitochondrial DNA repair.
• Localizes to the nucleus, nucleolus, nucleoplasm, and mitochondrion.
• Subject to acetylation, methylation, and phosphorylation, with a molecular weight of ~43 kDa (calculated 42.6 kDa).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within amino acids 250–350; consider this when assessing reactivity with isoforms or cleavage fragments.
• For Western blot (WB), expect a band near 43 kDa; include appropriate lysis controls and validate loading.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; optimize conditions and include tissue controls to confirm specificity.
• For ELISA, test antigen coating and antibody concentrations in relevant sample matrices; pairing with a detection antibody may enhance performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with a one-stop sourcing solution for IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this FEN-1 Rabbit mAb. Researchers and developers working on DNA replication and repair can rely on our supply chain for bulk ancillary reagents, rare target raw materials, and technical consulting to streamline assay development from concept to clinic.