Anti-FEN-1 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P39748

Core Product Specifications and Parameters

Biological Background: FEN-1 Function and Localization

• FEN-1 (Flap endonuclease 1), also known as DNase IV, MF1, or RAD2, is a structure-specific nuclease with both 5'-flap endonuclease and 5'-3' exonuclease activities.
• Plays a critical role in DNA replication by cleaving 5'-overhanging flap structures generated during Okazaki fragment processing, leaving a nick for ligation.
• Participates in the long patch base excision repair (LP-BER) pathway, cleaving within AP site-terminated flaps.
• Acts as a genome stabilization factor by preventing flaps from equilibrating into structures that could cause duplications and deletions.
• Also exhibits 5'-3' exonuclease activity on nicked or gapped double-stranded DNA and possesses RNase H activity.
• Involved in replication and repair of ribosomal DNA (rDNA) and mitochondrial DNA.
• Subcellular localization is predominantly nuclear, including the nucleolus and nucleoplasm, but mitochondrial localization has also been reported.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which may regulate its function and interactions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 50-380 of human FEN-1, covering the majority of the protein, which may contribute to recognizing multiple epitopes.
• For Western blot (WB), expect a band at approximately 43 kDa; consider using whole cell lysates from human or mouse cell lines.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), nuclear staining is anticipated, but mitochondrial localization may also be observed; optimize fixation and permeabilization conditions.
• In immunoprecipitation (IP) and ELISA, the polyclonal nature may enhance capture efficiency; validate with appropriate positive and negative controls.
• Cross-reactivity with mouse has been confirmed; for other species, perform sequence alignment and experimental validation before use.
• Store at -20°C as recommended for long-term stability; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including validated antibody pairs and polyclonal antibodies like this anti-FEN-1 antibody for DNA replication and repair studies. We also offer bulk ancillary reagents and specialize in sourcing rare-target raw materials to streamline your assay development from concept to clinic. Contact us to discuss custom sourcing and technical support.