Anti-FDXR Rabbit Polyclonal Antibody for WB, ELISA - P22570

Core Product Specifications and Parameters

Biological Background: FDXR Function and Localization

• FDXR encodes NADPH:adrenodoxin oxidoreductase, a mitochondrial flavoprotein also known as ferredoxin reductase. It belongs to the ferredoxin--NADP(+) reductase family and is involved in electron transfer processes.
• The primary function of FDXR is to serve as the first electron transfer protein in all mitochondrial P450 systems, including cholesterol side chain cleavage in steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.
• FDXR acts as a ferredoxin--NADP(+) reductase essential for coenzyme Q biosynthesis by transferring electrons to FDX2, which are required for the hydroxylation reaction performed by COQ6. Related references: PMID:38425362
• The protein is localized to the mitochondrion inner membrane, where it mediates electron transport from NADPH to adrenodoxin.
• FDXR contains FAD and NADP cofactors, and its activity is regulated by phosphorylation. It has been associated with diseases such as deafness, neuropathy, and neurodegeneration (based on UniProt keywords).
• Multiple isoforms exist due to alternative splicing, potentially influencing its subcellular distribution and functional diversity.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (a.a. 252–491), downstream of the predicted mitochondrial transit peptide, which may enhance specificity for the mature mitochondrial form.
• For Western blot, the expected band size is approximately 54 kDa (observed) / 53.8 kDa (calculated). Validate in relevant tissue lysates known to express FDXR, such as adrenal, kidney, or liver.
• This antibody is reported to react with human, mouse, and rat FDXR. Consider sequence alignment to verify epitope conservation across species for cross-reactivity validation.
• For ELISA, consider using the antibody as a capture or detection reagent with appropriate optimization for your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for sourcing high-quality IVD raw materials, from validated antibodies to bulk reagents, supporting mitochondrial research and steroid metabolism studies. This FDXR rabbit polyclonal antibody is part of our expanding catalog, and we also offer sourcing for rare targets and ancillary reagents to accelerate your diagnostic development. Explore our portfolio for optimized reagents covering every stage from concept to clinic.