Anti-FDX1/ADX Rabbit Polyclonal Antibody for WB, ELISA - P10109

Core Product Specifications and Parameters

Biological Background: FDX1/ADX Function and Localization

• Adrenodoxin (FDX1/ADX), also known as adrenal ferredoxin or hepatoredoxin, is a mitochondrial [2Fe-2S] ferredoxin that serves as an essential electron transfer protein for cytochrome P450 enzymes involved in steroid hormone biosynthesis.
• Essential for the synthesis of various steroid hormones; transfers electrons from adrenodoxin reductase to CYP11A1, which catalyzes the side-chain cleavage of cholesterol to pregnenolone — the rate-limiting step of steroidogenesis.
  Related references: PMID:20547883, PMID:21636783
• Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but instead functions as a mobile shuttle, sequentially interacting with each enzyme to transfer electrons (By similarity).
• Localizes to the mitochondrial matrix, consistent with its role in mitochondrial steroidogenic reactions.
• Highest expression is detected in the adrenal gland (at protein level); also present in kidney and testis.
• Contains one [2Fe-2S] cluster critical for its redox activity and electron transport function. Subject to post-translational modifications including acetylation and phosphorylation, which may modulate its activity or interactions.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the predicted molecular weight of human FDX1/ADX is approximately 19 kDa. Use reducing conditions and validate detection in mitochondrial-enriched fractions from appropriate human cell lines (e.g., adrenal, testis) for optimal signal.
• For ELISA, this polyclonal antibody can be used for capture or detection. Optimization of coating antigen concentration, blocking conditions, and detection antibody pairing is recommended based on the specific assay format.
• This antibody was raised against human FDX1/ADX (aa 1–184) and shows reactivity with human samples. Cross-reactivity with other species has not been determined; verify if intended for non-human applications.
• Because it is a polyclonal preparation, lot-to-lot consistency may vary; consider including appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, providing diagnostic manufacturers and labs with reliable access to critical reagents. Our FDX1/ADX rabbit polyclonal antibody supports research in steroidogenesis and mitochondrial biology. We also offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, helping streamline your assay development from early R&D to clinical application.