Anti-FDX1/ADX Polyclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - P46656

Core Product Specifications and Parameters

Biological Background: Adrenodoxin (Ferredoxin-1) Function and Localization

• Adrenodoxin, also known as ferredoxin-1, is a small iron-sulfur protein encoded by the FDX1 gene in mammals.
• It functions as a mobile electron carrier in the mitochondrial matrix, shuttling electrons from adrenodoxin reductase to mitochondrial cytochrome P450 enzymes.
• A key role is in steroidogenesis: it transfers electrons to CYP11A1, which catalyzes the first and rate-limiting step in steroid hormone biosynthesis (cholesterol side-chain cleavage).
• The protein contains a [2Fe-2S] cluster that is essential for its electron transfer activity; it does not form a ternary complex but shuttles between reductase and P450.
• Subcellular localization is strictly mitochondrial matrix, where it participates in steroid and lipid metabolism.
• Post-translational modifications include acetylation and phosphorylation, and the protein is synthesized as a precursor with a transit peptide that is cleaved upon mitochondrial import.
• Beyond electron transport, it is implicated in broader mitochondrial iron-sulfur cluster biogenesis and cellular redox homeostasis.
• The FDX1 gene is highly conserved across species, and its product is predominantly expressed in steroidogenic tissues such as the adrenal cortex and gonads.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region of mouse FDX1, which shares high homology with human and rat, supporting cross-reactivity.
• For Western blot (WB), the predicted molecular weight is approximately 20 kDa; consider using a 12–15% SDS-PAGE gel for optimal resolution.
• In immunoprecipitation (IP) and immunofluorescence (IF/ICC), ensure mitochondrial integrity is maintained during sample preparation; brief fixation may improve antigen accessibility.
• For immunohistochemistry (IHC-P), antigen retrieval methods such as heat-induced epitope retrieval in citrate buffer (pH 6.0) may enhance detection.
• The antibody can be used in ELISA; titrate the coating antigen and antibody concentration to achieve a linear detection range.
• Validate the antibody in the relevant species and tissue type, particularly in steroidogenic tissues like adrenal gland, testis, or ovary.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio streamlines sourcing for diagnostic developers and researchers, covering every stage from concept to clinic. This anti-FDX1/ADX antibody supports investigations into steroid metabolism and mitochondrial disorders, and is part of our extensive portfolio of validated polyclonal antibodies, optimized reagents, and bulk ancillary materials. For projects requiring rare targets or customized antibody pairs, CamelBio provides reliable procurement and technical consultation to accelerate your assay development.