Anti-FDPS Rabbit Polyclonal Antibody for WB, ELISA - P14324

Core Product Specifications and Parameters

Biological Background: FDPS Function and Localization

• FDPS (Farnesyl Pyrophosphate Synthase), also known as FPS, FPPS, or POROK9, is a key enzyme in the isoprenoid biosynthesis pathway that catalyzes the formation of farnesyl diphosphate (FPP) from dimethylallyl pyrophosphate and isopentenyl pyrophosphate.
• FPP is a critical precursor for the synthesis of sterols (including cholesterol), dolichols, carotenoids, and ubiquinones, and also serves as a substrate for protein prenylation (farnesylation and geranylgeranylation), which regulates the membrane association and function of many proteins, including small GTPases.
• The enzyme catalyzes two sequential condensation reactions: first condensing isopentenyl pyrophosphate with dimethylallyl pyrophosphate to form geranyl pyrophosphate, and then condensing a second isopentenyl pyrophosphate with geranyl pyrophosphate to yield farnesyl pyrophosphate.
• FDPS localizes predominantly in the cytoplasm, consistent with its role in the mevalonate pathway.
• Post-translational modifications include acetylation and hydroxylation, and the protein exists in multiple isoforms due to alternative splicing.
• The enzyme requires divalent metal ions (e.g., magnesium) as cofactors for its catalytic activity, and it is involved in host-virus interactions (e.g., with certain viruses that exploit the host prenylation machinery).
• Dysregulation or mutations in FDPS are associated with metabolic disorders and diseases, and it is a target for bisphosphonate drugs used in treating bone diseases.

Experimental Guidance and Technical Tips

• The immunogen covers a region spanning amino acids 120 to 419 of human FDPS; consider performing sequence alignment to confirm cross-reactivity with your species of interest (human, mouse, rat cross-reactivity is indicated).
• For Western blot, a band around 48 kDa is expected; optimal primary antibody dilution and incubation conditions should be empirically determined for your specific sample and detection system.
• ELISA may be used to detect native or recombinant FDPS; validate the assay in the relevant sample matrix and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers and laboratories from concept to clinic. This anti-FDPS polyclonal antibody supports research in metabolic disorders, cancer biology, and lipid signaling. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing assistance for rare target raw materials to streamline your assay development and production workflows.