Anti-FCN2 Polyclonal Antibody for WB, ELISA - Q15485

Core Product Specifications and Parameters

Biological Background: Ficolin-2 Function and Localization

• FCN2 is a calcium-dependent lectin that functions as a pattern recognition receptor, initiating the lectin pathway of complement activation to promote pathogen clearance and adaptive immune signaling. Related references: PMID:10679061, PMID:14707097, PMID:15804047

• Upon binding to carbohydrate moieties on pathogen surfaces, FCN2 activates the MASP1 serine protease, triggering the proteolytic complement cascade. Related references: PMID:14707097

• FCN2 specifically recognizes N-acetylglucosamine (GlcNAc), phosphocholine, and lipoteichoic acid, enabling broad-spectrum pathogen detection. Related references: PMID:14707097, PMID:17215869, PMID:25344472

• FCN2 enhances phagocytosis of Salmonella typhimurium by neutrophils, indicating an opsonic role. Related references: PMID:8576206

• FCN2 is a secreted protein found in plasma and on the cell surface, primarily produced by the liver.

• The protein contains collagen-like and fibrinogen-like domains, with post-translational modifications including disulfide bonds, hydroxylation, and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 230–290) of human FCN2; consider this when assessing epitope recognition in your assay.
• For Western blot, expect a band near 34 kDa under reducing conditions; validate using positive controls such as human serum or liver extracts.
• The antibody shows cross-reactivity with mouse and rat FCN2, likely due to sequence conservation; verify binding in your specific sample system.
• For ELISA, titrate the antibody to optimize the signal-to-noise ratio; note that polyclonal antibodies may exhibit lot-to-lot variation.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio offers high-quality immunological reagents like this anti-FCN2 polyclonal antibody for innate immunity and complement research. Beyond single antibodies, we supply validated antibody pairs, bulk ancillary reagents, and source rare targets to streamline your diagnostic development from concept to clinic.